Fig. 1

The effect of dietary methionine restriction on mTOR signaling and cancer progression. A Schematic of experimental design using HCC PDX model. Treatment, n = 5 mice per group. B Tumour growth curves and images of tumours at the end point (Day 28). C Survival analysis of mice under Ctrl or MR conditions. D Heatmap displaying global transcriptional changes of liver tissues between Ctrl and MR described in A. Red color represents the genes’ expression upregulated, while blue means the genes’ expression decreased. E KEGG pathway enriched for up-regulated and down regulated genes of liver tissues by comparing Ctrl to MR conditions. F Migration of SNU449 cells (left panel) with quantification data on the right panel. G Sphere formation efficiency of SNU449 cells (left panel) with quantification (right panel). H, I Tumour volumes and survival analysis of mouse allografts. n = 5 mice per group. Data are means ± SEM. Group differences were analyzed by two-tailed Student’s t test (B, C), two-way ANOVA followed by Tukey’s multiple comparison test (F, G, H) or log-rank test (I) (**p < 0.01, ****p < 0.0001). n.s., not significant. Ctrl, Control and MR, Methionine restriction