Fig. 3
From: Salidroside reduces neuropathology in Alzheimer’s disease models by targeting NRF2/SIRT3 pathway
SIRT3 is indispensable for SAL-mediated promotion of mitophagy. A qPCR shows the mRNA levels of SIRT3, 4 and 5 in SH-SY5Y cells treated with or without SAL. B Immunoblotting shows the levels of indicated proteins in SH-SY5Y cell treated by SAL with each indicated concentration. GAPDH as a loading control. C IF shows the colocalization of LC3-GFP and TOM20 in somata of SH-SY5Y cells with SIRT3 KD. Images in rectangles are amplified shown in lower panels. D As in C, except images of neurites are shown. Arrows indicate co-labeled yellow puncta. E Quantification shows the number of puncta co-labeled with LC3 and TOM20 in somata. F, G As in C, D, except SIRT3flox/flox cortical neurons transduced with AAV particles encoding mitoGFP and Cre were used and indicated as SIRT3 KO (see Additional file 1: Fig. S5). H As in E, except SIRT3 KO cortical neurons were used. Error bars indicates mean ± SD from at least 20 cells/neurites from 3 independent experiments (n ≥ 20). *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant; one-way ANOVA test. Scale bar, 10 μm