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Fig. 5 | Cell & Bioscience

Fig. 5

From: The balance between NANOG and SOX17 mediated by TET proteins regulates specification of human primordial germ cell fate

Fig. 5

Genetic Inactivation of DNMT3B Partially Rescues the hPGCLC Differentiation Defect of TKO hESCs. A ChIP–qPCR for DNMT3B at the NANOG and SOX17 promoters in WT and TKO hESCs; n = 3 independent experiments. Data are presented as means ± s.d. Statistical analysis was performed by Student’s t-test (two-sided), *p < 0.05; B Bright field and fluorescence images of Day 4 embryoid with BLIMP1-mKste2 reporter in WT, TKO, QKO hESCs, Scale bar = 100 μm; C FACS analysis for induction of hPGCs in WT, TKO, QKO hESCs; D Quantification of FACS at day 4 of hPGCLC induction in WT, TKO, QKO hESCs; n = 4 independent experiments. Data are presented as means ± s.d. Statistical analysis was performed by Student’s t-test (two-sided), * represent compared to WT group p < 0.05, # represent compared to TKO group p < 0.05; E Immunofluorescence of SOX17, TFAP2C, POU5F1, BLIMP1 and SOX2 at the day4 embryoid for WT, TKO and QKO cells. Scale bar = 50 μm; F RT-qPCR analysis for gene expression during hPGC differentiation in day 4 embryoid; n = 3 independent experiments. Data are presented as means ± s.d. Statistical analysis was performed by one-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001; G Methylation analysis of the NANOG and SOX17 promoters in hESCs and day 4 embryoids by Epimark; n = 3 independent experiments. Data are presented as means ± s.d. Statistical analysis was performed by one-way ANOVA: * represent compared to WT or WT_d4 group p < 0.05, # represent compared to TKO or TKO_d4 group p < 0.05

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