Fig. 1

A Sequence of images from a time-lapse recording showing how a THP-1 macrophage (green arrow) extends a lamellipodium (empty green arrow) to a wasteosome opsonized with ConA (red arrow) and pulls it towards the body of the macrophage, triggering the engulfment of the wasteosome. The phagocytosed wasteosome become later digested and fragmented (yellow arrows). Empty yellow arrow: wasteosome is out of the focus plane. See video for details. At 1202 min, confocal images were taken and the 3D reconstruction was made. B Sequence of images showing the 360° rotation of the 3D reconstruction. Images permit to observe the location of the remains of the wasteosome (red and yellow) at the macrophage. Some dots of red or yellow fluorescence appear on the surface of the macrophage, suggesting antigen presentation. The big green spot in the lower region corresponds to a macrophage that is in contact with the one that has phagocytosed and digested the wasteosome