Fig. 5

Acute activation response of ARC GLP-1R neuron on feeding behavior. A Experimental paradigm for virus delivery and chemogenetic stimulation of the GLP-1R neurons in ARC. B Food intake consumption upon activation of the GLP-1R neurons in ARC (0.5 g food fed overnight), p value = 0.0261*, control n = 7, hM3Dq n = 8. C Food intake consumption after 24 h CNO injection. D In an exploratory open field test, the dependent locomotor activity of control and hM3Dq mice after CNO delivery, measuring various parameters. E Active count. F Total active time (min). G Total traveled distance (cm). H Time spent in the cage center (min). Data are presented as mean ± standard error of the mean (SEM). Student’s t-test; *p < 0.05; **p < 0.01, ***p < 0.001. Two-way Anova, with Geisser-Greenhouse correction