Fig. 3

Expression of GLP-1R in the ARC and the effects of Exn4 on ARC neurons. A GLP-1 neuronal axonal projections in the ARC region. AAV-DIO-Chr2-EYFP was injected into the NTS region of the Gcg-Cre mouse. B GLP-1R-expressing neurons in the ARC region revealed by tdTomato fluorescence in GLP-1R-ires-Cre::Ai14 (LSL-TdTomato) mice. C Expression of GLP-1R in the ARC using a conditional viral-mediated approach. AAV-DIO-eGFP was injected into the ARC region of the GLP-1R-ires-Cre mouse. D Experimental paradigm for recordings from GLP-1R-expressing neurons labeled with tdTomato in GLP-1R-ires-cre::Ai14 tdTomato (Lox-Stop-Lox-TdTomato) reporter mice. E and F The resting membrane potential (RMP) of ARC GLP-1R-expressing neurons in the absence or presence of Exn4. Paired t-test, *p < 0.05, ***p < 0.001. G Pie chart of the proportion of ARC GLP-1R-expressing neurons showing RMP changes in the presence of Exn-4. H Represent traces of membrane potentials with action potentials (APs) in the ARC GLP-1R neurons with or without Exn4. I Pooled data. Recordings were conducted in 4 animals. Paired t-test, *p < 0.05. J and K Experimental paradigm for recordings in POMC-neurons. POMC-Cre mice were injected with AAV-DIO-eYFP in the ARC. L Representative traces of APs in POMC neurons in the absence or presence of Exn4. M and N Pooled data. Data are presented as mean + S.E.M., n = 5/2 cells/animals. Paired t-test, *p < 0.05