Fig. 5

TAMs promotes DNMT1 expression and cell motility in breast cancer cells through IL-6-pSTAT3-ZEB1-DNMT1 axis. A, B Assessment of cells motility by invasion assays (A) and migration assays (B) in MCF7-Vector/ZEB1 cells after treated with DMSO or P300 specific inhibitor A485 (60 nM). C–F ZEB1 and DNMT1 expression were analyzed by western blot assays (C, E) and qRT-PCR assays (D, F) after MCF7 cells were co-cultured with TAM-like medium or TAM-like U937 cells. G, H STAT3 and p-STAT3 expression were assessed by western blots in MCF7 cells after co-cultured with TAM-like medium or TAM-like U937 cells. I, J Western blots detection of ZEB1, DNMT1, p-STAT3 and STAT3 expression in MCF10A and MCF7 cells after treated with IL-6 (10 ng/mL, 48 h). K, L Assessment of ZEB1 and DNMT1 expression by qRT-PCR and western blot in MCF7 cells co-cultured with TAM-like medium after treated with STAT3 inhibitor WP1066 (2.43 μM). Data are represented as mean ± SD of three independent experiments, and *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test)