Fig. 2

DNMT1 promotes EMT progression and facilitates cells motility in breast cancer. A The morphological change of MCF10A cells was examined by phase constrast microscopy after overexpressing DNMT1. Scale bar: 100 μm. B–E Detection of EMT markers expression in mRNA level and protein level after overexpression of DNMT1 in MCF10A cells and MCF7 cells. F–I qRT-PCR assays and western blot assays analyzed EMT markers expression cells after knockdown DNMT1 or treated with DAC in MDA-MB-231 cells. J–O Assessment of cells motility by invasion assays (J, L, N) and migration assays (K, M, O) in MCF7-Vector/DNMT1 cells, MDA-MB-231-shCtrl/shDNMT1 cells, or MDA-MB-231 cells treated with DMSO/DAC. Data are represented as mean ± SD of three or four independent experiments, and *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t test)