Fig. 7
From: RNA binding protein HuR protects against NAFLD by suppressing long noncoding RNA H19 expression
Hepatocyte-specific HuR deficiency aggravates WDSW-induced hepatic fibrosis. HuRhKO and control mice were fed ad libitum a WDSW for 12 weeks. a Representative H&E staining images of the liver sections (scale bar, 20 μm for 40× magnification). b Relative mRNA levels of H19 in the liver of HuRhKO and control mice. c Representative images of the liver sections stained with F4/80, Picro Sirius Red staining, and IHC staining of CK19 (scale bar, 20 μm for 40 × magnification). d Relative mRNA levels of genes involved in hepatic fibrosis were determined by real-time RT–PCR and normalized with HPRT1 as an internal control. Relative mRNA levels of Ck19, α-Sma, Tgfβ, Lox12, Sox4, Sox9, Ctgf, Mmp2, Mmp7, Sctr, Postn, and S1pr2 are shown. Data are expressed as the mean ± SEM. Statistical significance relative to control: *p < 0.05, **p < 0.01, ***p < 0.001, n = 10–12.