Fig. 5
From: RNA binding protein HuR protects against NAFLD by suppressing long noncoding RNA H19 expression
HuR regulates the expression of SphK2 and S1PR2. a Representative immunoblot images of nuclear SphK2 in the livers of HuRhKO and control mice fed with WDSW for 4 weeks are shown. The relative protein level of SphK2 was calculated using histone H3 as a loading control. Statistical significance relative to control, *p < 0.05 (n = 3) b Representative immunoblot images of nuclear SphK2 in the livers of H19−/− and control mice fed with WDSW for 4 weeks are shown. The relative protein level of SphK2 was calculated using histone H3 as a loading control. Statistical significance relative to control, *p < 0.05 (n = 3). c Relative mRNA levels of S1PR2 in the liver of human NASH patients, WDSW-NASH mice, and HuRhKO mice fed with WDSW for 4 weeks, respectively. Statistical significance relative to control, **p < 0.01. d Representative immunoblot images of S1PR2 in the liver are shown and normalized with β-actin as an internal control of HuRhKO mice fed with WDSW for 4 weeks. Statistical significance relative to control, *p < 0.05 (n = 3). e Effect of S1PR2 on H19 promoter activity. HEK-293 cells were transfected with pGL3-human H19-promoter or pGL3-control vector together with pcDNA3-hS1PR2 or control vector along with Renilla control vector. After transfection for 48 h, the luciferase activities were measured using a dual-luciferase reporter assay kit. The promoter activity was expressed by relative luciferase activity using the ratio of firefly luciferase activity to Renilla luciferase activity. Statistical significance relative to control, ***p < 0.001 (n = 5)