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Fig. 2 | Cell & Bioscience

Fig. 2

From: Generation of mitochondria-rich kidney organoids from expandable intermediate mesoderm progenitors reprogrammed from human urine cells under defined medium

Fig. 2

Establishment of 3D U-iKOs. A Schematic illustration of 3D U-iKOs. Colonies at day14 in Fig. 1 were picked and then aggregated as pellets or single cells in suspension and air–liquid interface (ALI) culture to form 3D U-iKOs at indicated time points in B. B Representative bright-field images of 3D U-iKOs as in A. Scale bars, 250 μm. C Immunostaining analysis of nephron segment-specific markers for podocytes (PODXL, nephrin, and WT1), proximal tubule (LTL), medial tubule (JAG1), distal tubule (PAX2, PNA and CDH1), and basement membrane (LAM). Scale bars, 50 μm

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