Fig. 6

SK current and the expression of SKs in POMC neurons. A The expression of SK3 in male and female POMC neurons. B Fluorescence for tdTomato (left) and bright-field illumination (right) of a recorded POMC neuron in a brain slice. C Representative traces of SK current in male and female POMC neurons. D Quantification of SK current in POMC neurons from fed male and female mice. E Fluorescence for ZsGreen (left) and tdTomato (middle) and bright-field illumination (right) of a recorded POMC + ERα + neuron in a brain slice. F Representative traces of SK current in male and female POMC + ERα + neurons. G Quantification of SK current in POMC + ERα + neurons from male and female mice. H RNAScope using probes targeting POMC, SK3 and ERα in the ARH. White arrow indicated the neurons that co-express genes that encode POMC, SK3 and ERα. I Quantification of the percentage of POMC + SK3 + ERα + neurons out of total POMC neurons in the ARH. Data are presented as mean ± SEM with individual data points. N = 22–32 neurons per group. *, ****, P < 0.5 or 0.0001 in two-tailed unpaired t-test