Fig. 3

POMC specific deletion of SK3 decreased not only feeding but also physical activity in male mice, resulting in unchanged body weight. A-C Body weight (A), body mass (B) and food intake (C) of male control mice and pomc-SK3 KO mice. D-H O₂ consumption (D), CO₂ production (E) and heat production (F) of male control mice and pomc-SK3 KO mice. Left panel: temporal levels of each parameter measured by the TSE PhenoMaster during the dark cycle and light cycle within 24 h. Middle panel: ANCOVA analysis using body weight as a covariate (p value as indicated). Right panel: predicted O₂ consumption, CO₂ production or heat production using CalR analysis 35. Although two-way ANOVA did not reveal a significant difference, two-tailed unpaired t-tests detected several significantly different time points. G, H Physical activity (X + Y axis) (G) and physical activity (Z axis) (H) of male control mice and pomc-SK3 KO mice. Data are presented as mean ± SEM with individual data points. N = 6–14 mice per group. #P < 0.05 in two-way ANOVA analysis. *P < 0.05 in two-tailed unpaired t-test