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Fig. 6 | Cell & Bioscience

Fig. 6

From: Discovery of an autophagy inducer J3 to lower mutant huntingtin and alleviate Huntington’s disease-related phenotype

Fig. 6

J3 alleviate HD-associated biomarker and phenotypes of the HdhQ140 mouse model. AB The expression of T-HTT (anti-HTT, MAB2166) and DARPP-32 in the striatum of 15-month-old mice for homozygous HdhQ140/Q140 with or without 30 mg/kg of J3 administration for 3 months (A), and the protein level was quantified (B). Data are presented as mean ± sem from three individual samples. C The DAB staining of T-HTT (anti-HTT, MAB2166), DARPP-32, and Ub of striatum of indicating groups at 15-month-old. Protein expression level was quantified. Data are presented as mean ± sem from three individual samples. Scale bar = 100 μm. D Times of lifting at least two limps in open-field test for 15 min at 15-month-old for indicated groups. E The evaluation scores indicated phenotypes of the mouse in climbing test. FI Balance beam test of the slip faults times of three types of mice in 15 mm square (F), 15 mm round (G), 10 mm square (H), and 10 mm round (I). (JK) Rotarod test of the latency time of indicating groups at speed of 4 rpm (J), and 4 ~ 8 rpm (K) in 2 min. L 6 mg/kg of J3 was intravenous injected into 10-week-old mice. Plasma and brain concentrations of J3 were detected at 30 min. Each dot represented an individual mouse, and the bar represented mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001

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