Fig. 5

Comparison of functional studies of SSEA-1⁺ and SUSD2⁺ cells. a Representative images showing HUVECs tube formation in SSEA-1⁺-CM and SUSD2⁺-CM. CM, conditioned medium. b Graph on tube formation for HUVECs incubated with SSEA-1⁺-CM and SUSD2⁺-CM (Error bars represent s.d.; n = 3 technical replicates; two-tailed unpaired t-test, ***P < 0.001). c Analysis of gene set enrichment analysis (GSEA) highlighting the upregulation of in SSEA-1⁺ cells (NES = 2.189 of the VEGFA-VEGFR2 signaling pathway). d VEGFA in supernatant of SSEA-1⁺ and SUSD2⁺ cell analyzed by enzyme-linked immunosorbent assay (n = 6,*** P < 0.001). e qPCR analyses for the expression of VEGFA, TNF, IL-1A and IL-1B in SUSD2⁺ cells and SUSD2⁺ cells. Expression normalized to β-actin (n = 3 donors, two-tailed unpaired t-test, ***P < 0.001). f Representative images of wound healing assay of endometrial stromal cells carried out in SSEA-1⁺ and SUSD2⁺ coculture groups at 0 h, 12 h and 24 h. Scale bars, 100 µm. g Bar charts of relative wound healing from the experiments (n = 3 donors, two-tailed unpaired t-test, **P < 0.01, ***P < 0.001). h Analysis of GESA revealed the upregulation of cell adhesion in SSEA-1⁺ cells (NES = 1.627 of the cell adhesion molecules cams pathway. The following heatmaps show the subsets of enriched genes involved in their corresponding pathway. (i) qPCR analyses for the expression of ITGB2, ITGB7, E-cadherin and EpCAM in SSEA-1⁺ cells and SUSD2.⁺ cells, respectively (n = 3 donors, two-tailed unpaired t-test, **P < 0.05, ***P < 0.001)