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Fig. 3 | Cell & Bioscience

Fig. 3

From: Long-term maintenance of human endometrial epithelial stem cells and their therapeutic effects on intrauterine adhesion

Fig. 3

SSEA-1+ cells exhibit endometrial epithelial stem cell phenotype. a Overview of the protocol for SSEA-1+ and SUSD2+ cells differentiation into EEC-like cells. b Morphologic change of SSEA-1+ and SUSD2+ cells after differentiation. Scale bars, 100 µm. SSEA-1+/SUSD2+ Diff, SSEA-1+/SUSD2+ cells differentiation. c Flow cytometric analysis showing the proportion of EpCAM and CD9 positive-cells in SSEA-1+ or SUSD2+ cells at day 0 and day 20 of differentiation. Red, positive-cells; blue, negative controls. d Immunofluorescence analyses demonstrating the expression of Cytokeratin and Vimentin in EEC-like cells differentiated from SSEA-1+ and SUSD2.+ cells. e Gene expression levels for EpCAM, CD9, OCT-4, SOX2 and NANOG. Expression normalized to β-actin (two-tailed unpaired t-test, Error bars represent s.d., n = 3; n.s., non-significant; *P < 0.05, **P < 0.01, ***P < 0.001)

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