Fig. 3

Klf7 influences rhythmic expression of core clock genes. A Klf7 and Clock mRNA levels measured by qPCR in klf7 knockdown cells. The Clock mRNA level was decreased significantly compared with control group (n = 4). B Clock protein levels measured by western blot in klf7 knockdown cells. Clock protein levels consist with mRNA level (n = 5). C Rhythmic expression of core clock genes in control normal cells and klf7 knockdown cells (n = 4). Rhythmic expression of Clock was disrupted and the amplitude increased (n = 4). CT0 is the onset at hour 0 of subjective light period. The data are presented as the mean ± SEM. Statistical analysis (P^** < 0.01 and P^*** < 0.001) between control group and knockdown group in (A, B) were performed by unpaired t test. Statistical analysis (P^* < 0.05, P^** < 0.01, P^*** < 0.001 and P^**** < 0.0001) between control group and klf7 knockdown group in (C) were performed by one-way ANOVA test