Fig. 7
From: YAP1 protects against septic liver injury via ferroptosis resistance
YAP1 inhibited ferritinophagy by disrupting the NCOA4–FTH1 interaction. A LO2 cells in 4 groups were transfected with LC3-GFP plasmid, while the autophagosome in cells presented green fluorescence puncta. Representative confocal images of cells exhibited fluorescent colocalization (yellow puncta) of ferritin (red fluorescence) with LC3-GFP (green puncta). B Fluorescence quantification of autophagosomes (LC3-GFP) and ferritin colocalizing with autophagosomes, n = 3. C Immunoprecipitation analysis of NCOA4 and FTH1 interaction in LO2 cells. IgG was used for control. Data are expressed as mean ± SD of three replicates, *p < 0.05, **p < 0.01, ***p < 0.001