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Fig. 5 | Cell & Bioscience

Fig. 5

From: YAP1 protects against septic liver injury via ferroptosis resistance

Fig. 5

YAP1 activation inhibited ferroptosis by decreasing the amount of intracellular free ferrous iron that accumulated in mitochondria. LO2 cells transfected with YAP1 overexpression (YAP1 OE) clone lentiviral particle to constitute YAP1 OE cell lines. A MitoTracker (green) and Ferro Orange (red) were showed by co-staining in LO2 cells stimulated with LPS. Ferro Orange (red) exhibited the localization of intracellular free iron in living cells. The mitochondria were stained by Mito Tracker Green fluorescence. B Quantification of the fluorescence intensity of Ferro Orange colocalized with MitoTracker (green) and Ferro Orange fluorescence intensity, n = 3. C, D Representative images of Mitochondrial ROS stained by Mito-SOX and Quantitative results of Mitochondrial ROS, n = 3. E, F The expression levels of GPX4, ACSL4 and SFXN1 were showed by WB, n = 3. Data are expressed as mean ± SD of three replicates. *p < 0.05, **p < 0.01, ***p < 0.001

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