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Fig. 6 | Cell & Bioscience

Fig. 6

From: Aberrant methylation of Serpine1 mediates lung injury in neonatal mice prenatally exposed to intrauterine inflammation

Fig. 6

Serpine1 expression was regulated by TNF-α-Tet1 pathway. A, B DNA methylation levels analysed on the CpG island of the Serpine1 promoter using bisulfite sequencing PCR in lung tissues of neonatal mice. C-E Serpine1 expression analysed using RT-qPCR and western blot in 5-Aza-CdR-treated MLE-12 cells. F, G Tet1 expression in lung tissues of neonatal mouse determined using immunohistochemistry. Scale bars, 200 μm. H, I Tet1 expression in TNF-α-treated MLE-12 cells determined using immunofluorescence (green, DAPI: blue). Scale bars, 50 μm. J The effects of Tet1 on the transcriptional efficiency of the Serpine1 promoter using luciferase assay. Data show mean ± SEM; Data was analysed using unpaired t-tests and one-way ANOVA

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