Fig. 5

Serpine1 promoted the senescence of A549 cells. A, B Proliferation analysed using the EdU assay (green, DAPI: blue) in Serpine1-overexpressing A549 cells. Scale bars, 200 μm. C, D SA-b-gal activity measured through X-gal in Serpine1-overexpressing A549 cells. Scale bars, 200 μm. E, F Cell cycle of Serpine1-overexpressing A549 cells analysed using flow cytometry. Cell fractions in the G0/G1, G2/M, and S phases were calculated. G Seripine1, CCND1, CDK2, and CCNE2 expressions analysed using western blot. H The intracellular ROS content in Serpine1-overexpressing A549 cells measured using the fluorescent probe DCFDA. I JC-1 staining in Serpine1-overexpressing A549 cells. The JC-1 red/green signal ratio was calculated using a fluorescence plate reader. Data show mean ± SEM; Data was analysed using unpaired t-tests