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Fig. 4 | Cell & Bioscience

Fig. 4

From: Aberrant methylation of Serpine1 mediates lung injury in neonatal mice prenatally exposed to intrauterine inflammation

Fig. 4

Serpine1 promoted the senescence of MLE-12 cells. A, B Cell cycle of Serpine1-overexpressing MLE-12 cells analysed using flow cytometry. Cell fractions in the G0/G1, G2/M, and S phases were calculated. C, D CCND1, CDK2, CCNE2, and p16 expressions analysed using western blot in Serpine1-overexpressing MLE-12 cells. E The intracellular ROS content in Serpine1-overexpressing MLE-12 cells measured using the fluorescent probe DCFDA. F, G JC-1 staining in Serpine1-overexpressing MLE-12 cells. Scale bars, 50 μm. The JC-1 red/green signal ratio was calculated using a fluorescence plate reader. H, I γH2AX expression in Serpine1-overexpressing MLE-12 cells determined using immunofluorescence (green, DAPI: blue). Scale bars, 50 μm. Data show mean ± SEM; Data was analysed using unpaired t-tests

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