Fig. 3

Serpine1 decreased the proliferation of MLE-12 cells. A, B Serpine1 expression analysed using immunofluorescence and western blot in Serpine1-overexpressing MLE-12 cells. Scale bars, 50 μm. C, D Proliferation analysed using the EdU assay (green, DAPI: blue) in Serpine1-overexpressing MLE-12 cells. Scale bars, 25 μm. E, F Apoptosis rate analysed using flow cytometry in Serpine1-overexpressing MLE-12 cells. G, H SA-b-gal activity measured through X-gal in Serpine1-overexpressing MLE-12 cells. Scale bars, 200 μm. Data show mean ± SEM; Data was analysed using unpaired t-tests