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Fig. 5 | Cell & Bioscience

Fig. 5

From: Inhibition of sphingolipid metabolism in osteosarcoma protects against CD151-mediated tumorigenicity

Fig. 5

CD151 regulates the transcription of SPTLC1 by c-myc. a KEGG analysis for c-myc-related differentially expressed genes based on GEO database (GSE42352, n = 127). The red indicates the KEGG pathway related to sphingolipid metabolism. b and c SPTLC1 mRNA and protein levels in CD151 depletion cells stably infected with vector or c-myc-expressing lentiviruses were analyzed by RT-qPCR (b) and Western blot (c). Data are shown as mean ± SD of triplicate experiments. d and e Schematic representation of the promoter region in the human SPTLC1 gene (d). ChIP analysis from HOS-MNNG cells was performed with control IgG or c-myc antibody as indicated. The presence of the SPTLC1 binding was detected by qPCR using primers in either conver c-myc binding region (#3) or the negative unrelated region (#1, #2, and #4). Quantification of enrichments is represented as fold-enrichment over lgG control. f HOS-MNNG cells were transfected with an empty vector or c-myc plus the wild-type SPTLC1 promoter (WT) or mutated promoter (MUT) for measuring luciferase activity. Data are the mean ± SD, and the data are representative of three independent experiments

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