Fig. 6

INSM2 regulates fatty acid synthesis in NB cells. A Confocal images(left) of lipid droplet and quantification(right) in INSM2-knockdown SK-N-BE(2) cells. B Flow cytometry analyses of lipid droplet in SK-N-BE(2) cells stably transfected with sh-NC or sh-INSM2#1.DMSO and fatostatin treated SK-N-BE(2) cells were used as positive controls. C Volcano plot of LC/MS-MS–based lipidomics after INSM2 knockdown. Each dot represents a lipid species. D The lipid group Bubble Diagram showed the changes of lipid metabolites in INSM2-knockdown SK-N-BE(2) cells. Each dot represents a metabolite. E Schematic diagram showing the regulation of lipid synthesis pathways by INSM2 via integration of RNA-seq, and lipidomics data. Unpaired two-sided t-test was used for the analysis in a. ns, not significant;*P < 0.05; **P < 0.01. The data are based on three separate experiments