Fig. 5

INSM2 is involved in the regulation of mTORC1, Cholesterol and Adipogenesis signaling pathway. A Volcano plot showing differentially expressed genes in INSM2-knockdown SK-N-BE(2) cells. Genes with abs(log2(foldchange)) > 0.5 and adjusted p-value < 0.05 were considered significant. B–D GSEA plots show alterations in mTORC1, CHOLESTEROL and ADIPOGENESIS signaling pathways in INSM2-knockdown SK-N- BE(2) cells. E Heatmap showing the expression of cancerrelated genes differentially expressed in INSM2-knockdown SK-N-BE(2) cells. F WB showing that mTORC phosphorylation is inhibited after INSM2 knockdown. G qPCR analysis of mRNA levels for the genes related to fatty-acid synthesis in SK-N-BE(2) cells transfected with sh-NC or sh- INSM2#1. H Fatty acid synthesis related protein levels decreased. I Scatter plot showed correlations between INSM2 and FASN, ACC mRNA level in tumors of 88 NB patients investigated. Unpaired two sided t-test for analysis in G. NS, not significant; *P < 0.05; **P < 0.01. The data are based on three separate experiments