Skip to main content
Fig. 4 | Cell & Bioscience

Fig. 4

From: Super-enhancer-associated INSM2 regulates lipid metabolism by modulating mTOR signaling pathway in neuroblastoma

Fig. 4

INSM2 promotes NB growth in vivo. A Flowchart showing the experimental design used to obtain subcutaneous xenografts in nude mice; B Representative nude mice images of xenografts formed by subcutaneous injection of SK-N-BE(2) cells stably transfected with sh-NC or sh-INSM2#1 through the armpit of nude mice (n = 6). C Representative tumor images. D Weight growth curve of nude mice after injection. E Growth curves of tumor in vivo. F Tumor weight at the end points of xenografts(n = 6). G Western-blot of living tumor tissues. H–I IHC analysis for Cleaved Caspase-3 (H), Ki-67(I), expression was performed in sections of tumors harvested from xenografts, and percentages of the positive cells were quantified. All data are shown as mean ± SD (scale bars of H, I = 50 μm). The sh-NC group was compared to the sh-INSM2#1 group. One-way ANOVA with Bonferroni correction in D, E; unpaired two-sided t-test in F, H–I. NS, not significant; *P < 0.05; **P < 0.01. The data are based on three separate experiments

Back to article page