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Fig. 3 | Cell & Bioscience

Fig. 3

From: Super-enhancer-associated INSM2 regulates lipid metabolism by modulating mTOR signaling pathway in neuroblastoma

Fig. 3

INSM2 promotes survival and expansion of NB cells in vitro. A, B western blot analysis(A) and q-PCR (B) of INSM2 expression in SK-N-BE(2) and SK-N-SH cells infected with sh-NC or sh-INSM2s. GAPDH served as an internal reference. C Representative images of NB cells infected with sh-NC or sh-INSM2s. Scale bars: 200 μm. D-G SK-N-BE(2) and SK-N-SH cells were infected with sh-NC or sh- INSM2s. The cells were harvested for CCK-8 colorimetric assay (D), colony formation assay(E), cell apoptosis assay (F), periodic assay (G) after stable cancer cells were established. For A, D–G, representative images are shown in the left and quantification in the right. Each sh-INSM2 group was compared with the sh-NC group. One-way ANOVA with Bonferroni correction in D; unpaired two-sided t-test in A, E–G; ns, not significant; *P < 0.05, **P < 0.01. The data are based on three separate experiments

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