Fig. 1

Identification of INSM2 as a super-enhancer-associated gene in NB. A Enhancers were ranked by increasing H3K27Ac signal in 6 NB cell lines. The hockey stick plots shown genes associated with super-enhancers. B The ChIP-seq gene tracks represent the H3K27Ac signal in NB cell lines, and neural crest cells, while the last two tracks represent H3K4me3 and ATAC signals in BE(2)-C at the INSM2 gene loci. The y-axis represents the total number of mapped reads per million, while the x-axis represents the linear sequence of genomic DNA. C Activity of the dual luciferase reporter vector containing NC or super- enhancer motifs that were transfected into SK-N-BE(2) cells. D q-PCR of INSM2 expression in SK-N-BE(2) cells treated with GNE987 and MZ1. GAPDH served as an internal reference. Unpaired two-sided t-test was used for the analysis in C. ns, not significant; *P < 0.05; **P < 0.01. The data are based on three separate experiments