Fig. 4

Alveolar organoid formation from imPACs and imPAC2 cells. A Subconfluent imPACs and imPAC2 cells were infected with Ad-GFP for 16 h and collected for organoid formation in Matrigel as described in the Methods. Sphere formation was recorded under a fluorescence microscope at day 7 (a), day 14 (b), and day 21 (c). Representative images are shown. B SftpC expression in the organoids. The 21-day organoids were harvested for whole mount immunefluorescent staining with the primary antibody against SftpC (red). Cell nuclei were counterstained with DAPI. Representative images are shown. Staining without primary antibodies was used as a negative control (data not shown)