Fig. 6
From: Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer
TMPRSS2-ERG (e2e4) promotes docetaxel resistance and accelerates neuroendocrine process of prostate cancer. A Representative images and histogram of migration assays using LNCaP and C4-2 cells overexpressing TMPRSS2-ERG e1e4 or e2e4. B–C The CCK8 assay was used to measure cell viability in LNCaP and C4-2 cells when e1e4 or e2e4 was overexpressed. D The CCK8 assay was used to determine IC50 of enzalutamide in LNCaP cells with e1e4 or e2e4 overexpression. E Colony formation assay tested cell viability in C4-2 cells after docetaxel treatment when e1e4 and e2e4 were overexpressed. F The CCK8 assay was used to determine IC50 of docetaxel in C4-2 cells with e1e4 or e2e4 overexpression. G Gel images of RT-PCR product of e1e4 or e2e4 after using various concentrations of docetaxel treatment. H Representative image of the Western blotting analysis of CHGA, NSE and SYP levels after e1e4 or e2e4 overexpression in LNCaP and C4-2 cells. ****p < 0.0001, ***p < 0.001.**p < 0.01