Fig. 5
From: Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer
Validation of the TMPRSS2-ERG (e2e4) in cell lines and clinical samples. A Primers were designed based on the different exons of parental genes. B–D Gel images of Touch-down PCR products of the full length of TMPRSS2-ERG (e2e4) in NCI-H660 B, tumor mix samples C and adjacent normal mix samples D. It should be mentioned that the shallow band of R12-3 in tumor mix samples and R12 in adjacent normal mix samples are both non-specific amplifications confirmed by Sanger sequencing. E The schematic diagram of TMPRSS2-ERG (e2e4) translation, and the translated amino acid sequence. The sequence of extra coding region of TMPRSS2 is highlighted in green, and the sequence of extra coding region of ERG is highlighted in yellow. F Validation of protein coding potential of e1e4 and e2e4 plasmids using anti-Flag antibody and anti-ERG antibody