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Fig. 2 | Cell & Bioscience

Fig. 2

From: Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer

Fig. 2

Validation of chimeric RNAs. A Gel images of RT- PCR products of the 80 candidate chimeric RNAs, with red arrows pointing to the chosen bands for Sanger sequencing. B Sanger sequencing results of the validated chimeric RNAs, with red lines marking the junction sites. Forward primer was used for Sanger sequencing in FXYD2-DSCAML1, and reverse primer was used in TMPRSS2-ERG, EEF2-SLC25A42, and SNX13-ATP2C1. C Gel images of RT- PCR products of the 15 candidate chimeric RNAs, with red arrows pointing to the correct bands. PrEC: PrEC LH. Mix1: cDNA mix of LNCaP, C4-2, PC3 and DU145. Mix2: cDNA mix of NCI-H660 and LASCPC-01

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