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Fig. 9 | Cell & Bioscience

Fig. 9

From: PEX5R/Trip8b-HCN2 channel regulating neuroinflammation involved in perioperative neurocognitive disorders

Fig. 9

ZD7288 treatment increasing neuroinflammation and microglial activation of PND rats. A, Diagrammatic illustration of our findings. HCN2 is extensively expressed in different cells of the cortex and hippocampus, including neurons, microglia, and astrocyte. Sevoflurane down-regulated HCN2 mediating cognitive dysfunction, anxiety-like behaviors, microglial activation, and neuroinflammation. Sevoflurane combined with ZD7288 (HCN2 blocker) produced strong synergies in this process. B, ZD7288 treatment down-regulating HCN2 mRNA of PND rats (n = 4). C, ZD7288 treatment up-regulating CD68 mRNA of PND rats (n = 4). D, Immunoblotting of HCN2 and iba1(n = 3). E, ZD7288 treatment down-regulating HCN2 expression of PND rats. F, ZD7288 treatment activating microglia of PND rats. G, ZD7288 treatment increasing IL-6, IL-1β, and TNF-α in the hippocampus of PND rats (n = 4). H, ZD7288 treatment increasing IL-6, IL-1β, and TNF-α in the cortex of PND rats (n = 4). Data are shown as mean ± SD. *P < 0.05 or **P < 0.01.

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