From: Liver organoids: an in vitro 3D model for liver cancer study
Comparison | 2D cell lines | 3D organoids |
---|---|---|
Morphology | Sheet-like flat monolayer | Self-organization, mimicking organ structure |
Origin of cells | Cell line derived from a single cell | Related to tissues used for cell isolation |
Heterogeneity | Unable to recapitulate critical features of the native tissues | Able to recapitulate structural, genetic, transcriptional and histological features of the native tissues |
Resource costs | Low | High |
Long-term expansion | Immortalized and easy expansion | Robust long-term expansion with maintenance of heterogeneity |
Co-culture | Difficult; trans-well culture system allows co-culture of different cell types | Easy for co-culture of different cell types because of extracellular matrix |