Fig. 2

Hif-2α inhibits fatty acid β-oxidation in EtOH-treated AML-12 cells and EtOH-fed mice. EtOH-treated AML-12 cells were transfected with Hif-2α-siRNA or treated with PT2399 (2.0 μM) for 48 h, and mice fed EtOH liquid were intragastrically administered PT2399 (10 mg/kg) for 7 days. A, B Western blotting analysis of Hif-2α, and C, D CPT-1α and MCAD in Hif-2α-siRNA-treated and PT2399-treated AML-12 cells. E Representative Oil red o staining images of PT2399-treated AML-12 cells (scale bar = 100 μm), showing the triglyceride content in Hif-2α-siRNA- and PT2399-treated AML-12 cells (n = 3). F, G Representative Oil red o staining images and IHC-Hif-2α in PT2399-treated liver tissue (scale bar = 100 μm or 20 μm). H, I Western blotting analysis of Hif-2α, CPT-1α and MCAD in PT2399-treated liver tissue. J Serum triglyceride levels in PT2399-treated liver tissue (n = 6). Data are presented as the mean ± S.D. *P < 0.05, **P < 0.01