Fig.5
Effects of U87 expressing AQP4-tetramers or AQP4-OAPs-derived EVs on apoptotic activation of recipient U87 cells. a Representative image of U87 cells treated with 2 K and 10 K EVs derived from U87 expressing Mock, AQP4-tetramers or AQP4-OAPs as indicated and stained with DAPI to visualize Nuclei after 72 h of incubation. Scale bar 100 µm. b Dot plot showing the analysis of the nuclear area of U87 cells treated with 2 K and 10 K EVs derived from U87 expressing Mock, AQP4-tetramers or AQP4-OAPs. Values are expressed in µm2 and represent mean ± SEM. ****P < 0.0005, n = 3, two-way ANOVA/Tukey’s tests. c Epifluorescence images of U87 cells treated with 2 K and 10 K EVs-derived U87 expressing Mock, AQP4-tetramers or AQP4-OAPs after 72 h of incubation. The activity of Caspase-3/7 was detected by CellEvent™Caspase-3/7Green (green) detection reagent. Scale bar 100 µm. d Quantification of apoptotic cells in c. Results are expressed as the % of the positive cells per field and represent the mean ± SEM. **P < 0.005, ***P < 0.001, ****P < 0.0005, n = 4, two-way ANOVA/Tukey’s test