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Fig.4 | Cell & Bioscience

Fig.4

From: AQP4-dependent glioma cell features affect the phenotype of surrounding cells via extracellular vesicles

Fig.4

Effects of EVs derived from U87 expressing AQP4-tetramers or AQP4-OAPs on motility and viability of recipient U87 cells. a Representative images of U87 recipient cells treated with the 2 K and 10 K fractions of U87 transfected with Mock, AQP4- tetramers or AQP4-OAPs after 48 h migration into an agarose spot containing FBS. Lines denote the edge of the agarose spots. The arrows indicate the direction of chemoinvasion. Scale bar 500 µm. b Quantification of the maximum distance from the edge reached by cells reported in a, in the spot radius direction. Values are expressed as mean ± SEM ****P < 0.0001, ***P < 0.001, **P < 0.005 and *P < 0.05, n = 4, two-way ANOVA/Tukey’s tests. c MTT assay results showing U87 recipient cells viability after 72 h incubation with 2 K and 10 K EVs derived from U87 WT (Mock) and expressing AQP4-tetramers or AQP4-OAPs. The results are represented as a mean ± SEM, n = 3, two-way ANOVA/Sidak’s test

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