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Fig.2 | Cell & Bioscience

Fig.2

From: AQP4-dependent glioma cell features affect the phenotype of surrounding cells via extracellular vesicles

Fig.2

Formation of beaded apoptopodia by U87 cells expressing OAPs. a Phase contrast and Ethd-III staining (red) images of U87 WT and U87 expressing AQP4-tetramers or AQP4-OAPs as indicated. The arrows and arrowheads show apoptotic U87 cells expressing OAPs and derived apoptotic EVs, respectively. Scale bar 50 μm. b LEFT: Live phase contrast images of U87 expressing OAPs forming uniform beaded apoptopodia. Scale bar 10 μm. The boxed area is enlarged and the arrows indicate beads and relative diameters. Scale bar 5 μm; RIGHT: Quantitation of the diameter of vesicle-like structures on uniform beaded apoptopodia for 4 different fields of three independent experiments. c LEFT: Live phase contrast images of U87 expressing OAPs forming non-uniform beaded apoptopodia. Scale bar 10 μm. The boxed area is enlarged, and the arrows indicate beads and relative diameters. Scale bar 5 μm; RIGHT: Quantitation of the diameter of vesicle-like structures on non-uniform beaded apoptopodia for 4 different fields. The red shape indicates the diameter of the largest bead at the tip of each strand of apoptopodia. d Time-lapse images monitoring formation of individual vesicles through fragmentation of beaded apoptopodia. A series of time-lapse images taken at 10-min intervals is shown. The white arrows indicate the string. The red arrow indicates the release of vesicles from the string at the 60 min time point. Scale bar 20 μm. (see also Additional file1)

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