Fig. 4

Translational ERs of mentioned T3SS proteins were limited by total tRNA pools at later phase. A–C Twenty different tRNAs of four mentioned bacteria were detected by qRT-PCR with random primers. The cellular total tRNAs levels at various culture times in 1× T3SS-inducing conditions. The tRNAs of PS were detected at 8, 16, 24, 32, 40, 48, 60 min. D–L tRNAs of PA, XO and RS were detected at 10, 20, 30, 40, 50, 60 min. All experiments were repeated at least three times. The data at 0 min for each individual tRNA was set as 1. *p < 0.05; **p < 0.01; and ***p < 0.001. Results were indicated in mean ± SD. A–C showed tRNA-Ile, tRNA-Arg and tRNA-Asn as represents of P. syringae. D–F showed tRNA-Ile, tRNA-Val and tRNA-Asn as represents of P. aeruginosa. H–J showed tRNA-Phe, tRNA-Asp and tRNA-Gly as represents of X. oryzae. K–M showed tRNA-Ile, tRNA-Tyr and tRNA-His as represents of R. solanacearum. The standard deviations were shown but were very small in the plot