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Fig. 3 | Cell & Bioscience

Fig. 3

From: Maintenance of tRNA and elongation factors supports T3SS proteins translational elongations in pathogenic bacteria during nutrient starvation

Fig. 3

The first T3SS full-length protein synthesis back the full-length mRNA synthesis under 1× T3SS-inducing conditions. A–H Induction assay of full-length T3SS mRNAs (hrpLPS, hopG1PS, hrpRPS, hopX1PS, hrpA2PS, popNPA, hpa1XO and hrpGRS) of transcriptional and translational fusion reporters which were induced in 1× T3SS-inducing conditions. The qRT-PCR primers were used to detect the 3′ end region of T3SS mRNAs. qRT-PCR detected the synthesis kinetics of full-length T3SS mRNAs. The Schleif plots (\(\sqrt{E\left(t\right)-E(0)}\)) of all T3SS mRNAs were shown in red. The standard deviations were shown but were very small in the plot. All experiments were repeated at least three times

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