Fig. 7

CTCF and Tet1 coregulate the expression of Myc. A, B The expression of CTCF was significantly inhibited in iHepSCs by shRNA as detected by qRT-PCR (A) and Western blot (B) (n = 3, Student’s t test, ***P < 0.001). C, D The expression of Myc was reduced in CTCF-KD iHepSCs, as detected by qRT-PCR (C) and Western blotting (D) (n = 3, Student’s t test, **P < 0.01). E The results of ChIP-PCR showed that CTCF was enriched in the CBS-1 and site A regions but not in the TATA box and CBS-2 regions in iHepSCs (n = 6, Student’s t test, **P < 0.01). F Immunocytofluorescence staining with anti-CTCF antibody and anti-TET1 antibody revealed the colocalization of CTCF protein and TET1 protein in the nucleus. G Co-IP assay showed that anti-TET1 antibody and anti-CTCF antibody could pull down CTCF1 and TET protein, respectively, from the nuclear extract of iHepSCs