Fig. 6

Tet1 regulates the expression of Myc by directly binding to the CBS-1 and site A regions of the Myc promoter and demethylating the CpG cytosine. A Schematic diagram of the position of identified cis-elements that participate in the regulation of Myc expression on murine chromosome 15. Red rectangles represent CBS-1 and CBS-2. Blue arrows represent two initiating sites of transcription (P1, P2). Green rectangle indicates site A. The blue double-sided arrows represent the sites of primer sets used in Tet1-ChIP PCR. B ChIP-PCR revealed that TET1 was enriched in the CBS-1 and site A regions rather than the TATA box and CBS-2 regions in iHepSCs (n = 6, Student’s t test, ***P < 0.001). C Schematic diagram of methylated CpG cytosine in the sequence of CBS-1 and site A core region in MEFs, iHepSCs and Tet1 knockdown iHepSCs detected by BSP. The levels of methylation of cytosine in both regions of iHepSCs were lower than those of MEFs and Tet1-knockdown iHepSCs. The circles represent CpG dinucleotides. The filled circle is methylated cytosine, and the empty circle represents unmethylated cytosine. Ten clones of each sample were sequenced. Circles filled with dark blue ~ 100% positive; Circles filled with light blue 50–70% positive, empty circle ~ ≤ 30% positive. D The results of Pol II ChIP-PCR showed that the enrichment of Polymerase II at site A in iHepSCs was higher than that in MEFs. E The results of ChIP-PCR showed that the enrichment of H3K4me3 at site A in iHepSCs was higher than that in MEFs (n = 6, Student’s t test, **P < 0.01). F The results of ChIP-PCR showed that the enrichment of H3K27me3 in iHepSCs and MEFs had no difference (n = 6, Student’s t test)