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Fig. 5 | Cell & Bioscience

Fig. 5

From: Induced hepatic stem cells maintain self-renewal through the high expression of Myc coregulated by TET1 and CTCF

Fig. 5

Tet1 maintains self-renewal by targeting Myc in iHepSCs. A, B The expression of Myc was reduced after the expression of Tet1 was knocked down by shRNA, as detected by qRT-PCR (A) and Western blotting (B). C The level of 5hmC in iHepSCs treated with the indicated dosage of TET-IN-C35 for 6 days was analyzed by dot blot. Each sample was loaded with 500 ng genomic DNA. D The expression level of Myc in iHepSCs treated with TET-IN-C35 was measured by qRT-PCR analyses. E The overexpression of Myc recovered the proliferation capacity of Tet1-KD iHepSCs. F The overexpression of Myc increased the colony numbers of Tet1-KD iHepSCs. G The overexpression of Myc increased the sphere numbers of Tet1-KD iHepSCs. The data are shown as the mean ± SEM, n = 3, Student’s t test (A, B, F and G) and Dunnett’s test (D), *P < 0.05, **P < 0.01, ***P < 0.001, n.s., not significant

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