Fig. 4

Myc maintains the self-renewal of iHepSCs. A, B The expression of Myc in iHepSCs was higher than that in MEFs, as detected by qRT-PCR (A) and Western blotting (B). C, D The expression of Myc in iHepSCs and Myc knockdown iHepSCs was detected by qRT-PCR (C) and Western blotting (D). E The proliferation capacity of iHepSCs was inhibited after the expression of Myc was knocked down by shRNA. F The colony number of iHepSCs was significantly reduced after the expression of Myc was knocked down by shRNA. G The sphere numbers of iHepSCs were reduced after the expression of Myc was knocked down by shRNA. The data are shown as the mean ± SEM, n = 3, Student’s t test, **P < 0.01, ***P < 0.001