Fig. 3

Tet1 maintains the self-renewal of iHepSCs. A CCK 8 assay showed that the proliferation capacity of iHepSCs was inhibited after downregulating the expression of Tet1 with shRNA. B Representative images of colony formation of iHepSCs-scramble and Tet1-KD iHepSCs (left), and the numbers of colonies were significantly reduced in iHepSCs after downregulating the expression of Tet1 (right). C Representative plots (left) and statistical chart (right) of the percentage of G₀/G₁, S, and G₂M cells of iHepSCs-scramble and Tet1-KD iHepSCs. The cell cycle of Tet1-KD iHepSCs exhibited obviously arrested G₀/G₁ compared to iHepSCs-scramble. D Representative plots (left) and statistical chart (right) of apoptosis of iHepSCs-scramble and Tet1-KD iHepSCs. E Representative images of spheres of iHepSCs-scramble and Tet1-KD iHepSCs (left), and the numbers of spheres were significantly reduced in iHepSCs after downregulating the expression of Tet1 (right). The data are shown as the mean ± SEM, n = 3, Student’s t test, *P < 0.05, **P < 0.01