Fig. 2

Tet1 maintains a high level of 5hmC in iHepSCs. A The results of qRT-PCR showed that the expression of Tet1 mRNA was repressed by shRNA. B The expression of Tet1 protein was determined by Western blot. C The quantification of the relative intensities of blots (B) showed that the protein expression of Tet1 was significantly downregulated. D The results of dot blot showed that the level of genomic 5mC was upregulated when the expression of Tet1 was downregulated. Each sample was loaded with 500 ng genomic DNA. E The results of dot blot showed that the level of genomic 5hmC was downregulated when the expression of Tet1 was inhibited. Each sample was loaded with 500 ng genomic DNA. The data are shown as the mean ± SEM, n = 3, Student’s t test, **P < 0.01, ***P < 0.001