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Fig. 1 | Cell & Bioscience

Fig. 1

From: Induced hepatic stem cells maintain self-renewal through the high expression of Myc coregulated by TET1 and CTCF

Fig. 1

iHepSCs exhibit higher levels of 5hmC and Tet1 expression. A, B Immunocytofluorescence staining showed that the level of 5mC was much lower in iHepSCs than in MEFs (A), while the level of 5hmC was much higher in iHepSCs than in MEFs (B). C Dot blot assay showing the levels of genomic 5mC and 5hmC in MEFs and iHepSCs. D Tet enzyme activity increased dramatically in iHepSCs compared with MEFs. E The results of qRT-PCR showed that the expression level of Tet1 mRNA in iHepSCs was significantly higher than that in MEFs. F, G Western blot (F) and immunocytofluorescence staining (G) assays showed that Tet1 expression in iHepSCs was significantly higher than that in MEFs. The data are shown as the mean ± SEM, n = 3, Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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