Fig. 4

The binding between β-catenin and TCF family in SPCs. Expression of Tcf family was determined in SPCs using RT-PCR (M: marker; 1: testis; 2: SPCs; 3: negative control) (A). The expression of LEF1, TCF3 and TCF4 was detected in 42-day testis using IHC (SPCs populations residing in basal membrane were enclosed in red frames) (B). Co-IF staining demonstrated the colocalization of E-cadherin/LEF1 (C), E-cadherin/TCF3 (D) and E-cadherin/TCF4 (E) in purified SPCs. The binding between β-catenin and LEF1/TCF3/TCF4 was examined with co-IP (F). The expression of LEF1, TCF3 and TCF4 was determined in scrambled or β-catenin siRNA treated SPCs using Western blot (G). The expression of β-catenin and PLZF was detected in scrambled or Lef1/Tcf3 siRNA treated SPCs, respectively (H, I). Scale bar = 20 μm