Fig. 6

The effects of melatonin on retinal degeneration in NaIO₃-treated mice. C57BL/6 mice were pretreated with melatonin (25, 50 mg/kg) for 7 days and cotreated with NaIO₃ via intravenous injection. A After intravenous injection, changes in the optical coherence tomographic (OCT) images in the day 7-group mice were collected using a Micron IV camera. B Retinal histology for day 7 mice was detected by H&E staining and IHC staining. C The lengths of the ONL and IS/OS were quantified on the basis of retinal histology data. D Normal organ tissues were examined by H&E staining. E Proposed mechanism by which melatonin alleviates NaIO₃-induced ROS stress-mediated mitophagy through HIF-1α/BNIP3 modulation of LC3 expression in vitro and in vivo. All of the data are presented as the mean ± SEM of three independent experiments. **P < 0.01 compared with the control group and # P < 0.05 compared with the NaIO₃ treatment group